Ovarian cell-mediated immune response to Salmonella enteritidis infection in laying hens (Gallus domesticus).

نویسندگان

  • A Barua
  • Y Yoshimura
چکیده

The aim of this study was to examine the response of cell-mediated ovarian immunity against Salmonella infection in hens. Laying hens were injected intraperitoneally with PBS (control) or Salmonella enteritidis (SE). Ovarian stroma containing stromal follicles, small white follicles (SWF), and third largest (F3) and the largest (F1) follicles were collected 12 or 24 h after inoculation and fixed in periodate-lysine-paraformaldehyde. Frozen sections were stained first for CD3+, CD4+, or CD8+ T cells and then for SE by a double immunostaining method. Immunoreaction products for SE were detected in the ovarian stroma, theca of stromal follicles, SWF, F3, and F1 at 12 and 24 h after inoculation. Immunopositive T-cell subsets were localized in the stroma and theca of follicles in birds inoculated with or without SE. The populations of CD3+, CD4+, and CD8+ T cells were significantly greater in the stroma and the theca of follicles 12 h after SE inoculation than in those of control birds (P < 0.01). Their frequencies were further increased in those tissues 24 h after inoculation (P < 0.01). Injection of SE did not cause significant differences in the CD4+:CD8+ T-cell ratio as both subsets increased proportionately. The current results indicate that the population of T-cell subsets increases in the ovarian stroma and the follicular tissues in response to SE invasion within 12 h of inoculation. Thus, cell-mediated immune response against SE, their products, or both may be induced in the hen ovary.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A critical review of Salmonella Typhimurium infection in laying hens.

Salmonella Typhimurium has been reported to contaminate egg production across the world, but where Salmonella Enteritidis is endemic it is this latter serovar that dominates egg-borne salmonellosis. However, Salmonella Typhimurium is a major food-borne pathogen so it is important to understand how it can impact the microbiological safety of eggs and what serovar-specific control strategies may ...

متن کامل

Histology of the Ovary of the Laying Hen (Gallus domesticus)

The laying hen (Gallus domesticus) is a robust animal model for epithelial ovarian cancer. The use of animal models is critical in identifying early disease markers and developing and testing chemotherapies. We describe the microscopic characteristics of the normally functioning laying hen ovary and proximal oviduct to establish baselines from which lesions associated with ovarian cancer can be...

متن کامل

Immune response following vaccination against Salmonella Enteritidis using 2 commercial bacterins in laying hens.

The humoral and cell-mediated immune (CMI) response to 2 commercial killed Salmonella Enteritidis (SE) vaccines (Layermune and MBL SE4C) was evaluated in laying hens. Layers were distributed in 2 experimental groups. The first received a single immunization at 16 wk of age, while the second experimental group was immunized at 12 wk of age and again at 18 wk of age. Serum immunoglobulin (Ig)G an...

متن کامل

Colonization of specific regions of the reproductive tract and deposition at different locations inside eggs laid by hens infected with Salmonella enteritidis or Salmonella heidelberg.

Internal contamination of eggs by Salmonella Enteritidis has been a significant source of human illness for several decades and is the focus of a recently proposed U.S. Food and Drug Administration regulatory plan. Salmonella Heidelberg has also been identified as an egg-transmitted human pathogen. The deposition of Salmonella strains inside eggs is apparently a consequence of reproductive tiss...

متن کامل

Infection of the reproductive tract and eggs with Salmonella enterica serovar pullorum in the chicken is associated with suppression of cellular immunity at sexual maturity.

Salmonella enterica serovar Pullorum causes persistent infections in laying hens. Splenic macrophages are the main site of persistence. At sexual maturity, numbers of bacteria increase and spread to the reproductive tract, which may result in vertical transmission to eggs or chicks. In this study we demonstrate that both male and female chickens may develop a carrier state following infection b...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Poultry science

دوره 83 6  شماره 

صفحات  -

تاریخ انتشار 2004